In the arid and water-scarce Thar Desert of Rajasthan, traditional practices have played a vital role in water conservation for centuries. This study aims to explore the significance and effectiveness of these practices in sustaining water resources in the region. The research focuses on the intersection between spirituality, cultural practices, and effective environmental conservation in the Thar Desert. It delves into the concept of sacred groves, with a particular emphasis on the Kolu Pabuji Oran of Western Rajasthan. The study examines the biodiversity profiling of this sacred grove, highlighting its role in preserving the ecosystem and maintaining water balance. Furthermore, the investigation delves into the Teej Festival, a significant cultural event in Rajasthan, and its traditional water conservation practices. The festival serves as a platform to raise awareness about the importance of water resources and the need for sustainable practices. The study also explores various traditional water harvesting systems prevalent in the Thar Desert. It examines the Jhalara, a unique water conservation structure found in several districts of Rajasthan. Additionally, the research investigates the Talab/Bandhi, Bawaris, Taanka, and Johad, which are traditional water storage structures used to collect and store rainwater in different districts of the state. By analyzing these traditional practices, the study aims to shed light on their effectiveness, sustainability, and relevance in the context of modern water conservation efforts. It explores the potential for integrating traditional knowledge with contemporary methods to address the water challenges faced in the Thar Desert. Overall, this research contributes to our understanding of the role of traditional practices in water conservation in the Thar Desert of Rajasthan. It highlights the importance of preserving and revitalizing these practices to ensure the sustainable management of water resources in arid regions.