When individuals eat cabbage, carrots, spinach, tea, wheat and other Indian foods, fluoride is increasingly entering the human food and drink chain. Therefore, food fluoride concentration should not be ignored when estimating total fluoride intake. The amount of fluoride in food is influenced by the concentration of fluoride in soil and irrigation water. Therefore, utilizing sodium fluoride with different concentrations in the water used to irrigate the plant Amaranthus dubius, the current study evaluated the induced effects of water-soluble fluoride on protease enzyme activities from leaves, stems, roots and seeds. The findings demonstrated that on the 55th day of growth, Amaranthus dubius irrigated with 50 ppm sodium fluoride had a high amount of protease (4.82 µg/mg/5min) in its leaves and a lesser amount (0.06 µg/mg/5min) in its roots of the control, which receives only water. The amount of protease increases when concentration of sodium fluoride increases. Protease levels (µg/mg/5min) in the leaves of all experimentally challenged Amaranthus dubius ranged from 0.06µg/mg/5min to 4.82 µg/mg/5min (lowest in roots to maximum in leaves) on an expected time scale of 15 to 55 days.