The study was conducted to investigate the phytochemical profile and evaluate the in vitro anti-inflammatory activity of Blumea oxyodonta leaf extract. The methanol and ethanol extracts of B. oxyodonta leaves were found to contain bioactive compounds such as alkaloids, catechins, coumarins, flavonoids, phenols, glycosides, saponins, steroids, terpenoids, sugars, and xanthoproteins. The ethanol extract demonstrated notable anti-inflammatory effects by inhibiting protein denaturation, proteinase, heat-induced hemolysis, hypotonicity-induced hemolysis, and lipoxygenase activities, with inhibition percentages of 87.63%, 84.83%, 82.72%, 88.46%, and 80.73%, respectively, at a concentration of 1000 μg/mL. These results indicate that the ethanol extract of Blumea oxyodonta leaves has significant potential as an anti-inflammatory agent.