Background: The umbilical cord attaches the fetus to the placenta. Unhygienic cord care practices are an important cause of infection in the first few days of life. Therefore, care must be taken to prevent infection in the newborn by using properly sterilized instruments and cord tie for cord cutting and tying and cord must be kept dry and clean. Objective: The present study was undertaken to assess the knowledge and practices regarding umbilical cord care of the neonate in a rural community of Ghaziabad district. Methodology: The current study was carried out in Santosh Medical College, Ghaziabad from June 2018 to May 2020. The present study is a community-based cross-sectional study. Sample size is determined by Fisher’s formula and multi-stage sampling technique was used. Taking the safe upper limit allowing for non-responses (10%), a sample size of 300 was taken. For data collection a semi-structured study schedule was used as the tool. Result: This study was conducted among population of one month to six months of age constituting 51% females and 49% males. Out of total 300 study population, only 12.3% were aware of common symptoms of umbilical cord infection. This shows poor knowledge of umbilical cord stump infection in a newborn. While 49.7% mothers had knowledge that it prevents cord infection.87.7% mothers kept umbilical cord of the newborn clean. Conclusion: The study population had low cord care knowledge. Strategies need to be instituted by health care workers to improve knowledge of umbilical cord care and inappropriate practices should be strongly discouraged.