The analysis was conducted to identify the phytochemical components of the methanolic extract from the seed coat gel of Cassia fistula analysis by GC-MS. The phytocomponent analysis of the methanolic Cassia fistula seed coat gel extract by using agilant technology Gas chromatography- Mass spectroscopy. Chromatogram of methanolic extract of seed coat gel of cassia fistula showed the thirty seven peaks which having presence of phytochemical constituents. The phytochemical constituent highly present in extract are 1H-Imidazole (0.87%), 1-Methoxy-1-buten-3-yne (1.03%), 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-(1.72%), 2-Butenenitrile (21.8%), Decanoic acid, 3-methyl (1.52%), 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester (8.65%), Ethylvinyl sulfide (1.54%), m-Phenoxybenzoic acid (1.73%) Ethyl .gamma.-[1-naphthyl]butyrate (13.9%), 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione (1.7%), Myo-Inositol, 4-C-methyl- (0.81%), Myo-Inositol, 4-C-methyl- (5.11%), Dibutyl phthalate (2.35%),Ethyl hydrogen malonate (4.09%),2-Nitro-1-buten-3-ol (3.79%),Ethyl hydrogen malonate (6.89%),Methyl 4-O-methyl-d-arabinopyranoside (3.13%),Heptanoic acid (13.1%),6-Octadecenoic acid (0.74%). from thirty seven compounds were identified only ten were shows major biological activities in analysis gives that Cassia fistula seeds coat gel having different bioactive components.