The oil-in-water Nano emulsion (NE) for antidiabetic activity developed by the spontaneous emulsification method by using different ratio of span80, tween80, olive oil, acetone and water. The nano emulsion area was identified by constructing pseudo ternary phase diagrams by using different ratio of oil, surfactant/co-surfactant and water formulations were optimized. The nano emulsion formulations that passed tests were characterized for viscosity, droplet size, zeta potential, Polydispersity index, scanning electron microscopy and in vitro study was carried out in USP dissolution apparatus. The optimized nano emulsion formulation constituted as an oil phase, surfactant, and co-surfactant to restrain nanodroplet size and low viscosity and distilled water. In vitro dissolution studies showed higher drug release, finest droplet size, slightest polydispersity value, least viscosity and immeasurable dilution capability from the nano emulsion as compared with existing oral tablet formulation. The optimized RPG and MTF nano emulsion formulation showed better hypoglycemic effect in comparison to tablet formulation in experimental diabetic rats. No significant variations were also observed in the optimized formulation when subjected to accelerated stability study at different temperature and relative humidity.