Diabetes mellitus is a complex, irreversible metabolic illness that affects insulin secretion, function, or both, and raises blood sugar levels. Even now, acarbose and voglibose, either alone or in combination with insulin, are used as inhibitors of the enzymes that break down carbohydrates. Nevertheless, these substances have been linked to adverse side effects. Therefore, this study was conducted with the main purpose to evaluate the in-vitro antidiabetic potential of methanolic leaf extract of Aloe vera. Results depicted that the IC50 value of methanolic leaf extract of Aloe vera was found to be 8.94 mg/mL in alpha-amylase inhibition activity which was comparable with that of IC50 value standard antidiabetic drug acarbose (5.84 mg/mL). Similarly, IC50 value of methanolic leaf extract Aloe vera in alpha- glucosidase inhibition assay was found to be 9.93 mg/mL, and which was also comparable with that of standard antidiabetic drug acarbose (8.56 mg/mL). The total phenolic quantity was found to be highest (36.43 mgGAE/g extract) in methanolic leaf extract of Aloe vera when compared with total flavonoid quantities (1.68 mg QE/g extract). Inconclusion, this study demonstrated the anti-diabetic potential of methanolic leaf extract of Aloe vera. Hence, Aloe vera with the natural properties could be considered in for prevention of diabetes without any side effects.