IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

“Development and validation of an RP-HPLC method for the simultaneous estimation of praziquantel and ivermectin in bulk and combined tablet dosage form.”

Main Article Content

Mayur Atmaram Mahajan, Vaishali D. Rakibe, Sandip J. Sonawane, Nilesh K. Pathade, Amit B. Chaudhari

Abstract

A simple, rapid, accurate, and cost-effective RP-HPLC method was devised to estimate praziquantel and ivermectin in combination tablet dosage form. Chromatography was conducted using a Cosmosil C18 column (250 mm x 4.6 mm, particle size: 5 microns), with a mobile phase consisting of methanol and HPLC grade water in a ratio of 80:20 v/v, along with O-phosphoric acid to adjust the pH to 3. The flow rate was set at 1.0 ml/min, and the detection was done at 223 nm. The retention times were found to be 4.757 ± 0.10 min for praziquantel and 6.751 ± 0.10 min for ivermectin. The method shows a linear response when the concentration is between 25-125µg/ml for Praziquantel and 1-5µg/ml for Ivermectin. The method was validated using parameters such as accuracy, precision, linearity, limit of detection and quantitation as well as robustness.

Article Details