Peptic ulcers, characterized by erosions in the gastrointestinal mucosa, present a significant health challenge worldwide. While conventional pharmaceutical approaches have been effective in treating these ulcers, concerns regarding side effects and drug resistance persist. In light of this, the development and optimization of alternative, natural therapies, such as herbal formulations, have gained considerable attention. This research endeavors to create a novel herbal formulation tailored for the treatment of peptic ulcers. A systematic approach was followed, encompassing the selection and preparation of herbal ingredients renowned for their anti-ulcer properties, rooted in both traditional knowledge and contemporary scientific evidence. The formulation underwent rigorous optimization employing Design of Experiments (DoE) techniques to determine the ideal ratios and extraction methods for its constituents. This study signifies a crucial step towards the advancement of natural therapies for peptic ulcer treatment. By marrying traditional wisdom with contemporary optimization techniques, a novel herbal formulation has emerged as a potential alternative or adjunct to conventional pharmaceutical treatments. As peptic ulcers continue to pose significant clinical challenges, the development of effective and safe herbal formulations represents a promising avenue for improved patient care and underscores the importance of bridging traditional knowledge with modern scientific methodologies. Further clinical investigations are warranted to validate these findings and explore the practical applications of this herbal formulation in clinical settings.