The main objective of endodontic treatment is to prevent further infection or reinfection by eliminating microorganisms within the root canal system. Proper endodontic treatment could prevent apical and coronal penetration of fluids and microorganisms. Endodontic sealer is vital components of root canal obturation to establish a fluid-tight seal. Bioceramic-based root canal sealers are considered to be an advantageous technology in endodontics and have been found to be both biocompatible and comparable to other commercial sealers. The aim of this study was to compare the adhesion of three bioceramic sealers within the root canal system. Method: Endodontically treated teeth were obturated using three types of bioceramic sealers and then divided into three groups. Specimens were then observed using a scanning electron microscope, and the attachment distance was measured using ImageJ. Results: The three groups exhibited were statistically significant differences (p<0.05) in dentinal sealing ability. Calcium phosphate silicate-based sealer showed the highest sealing ability, followed by pure tricalcium silicate-based bioceramic sealers and then tricalcium silicate and resin-based bioceramic sealers. Conclusion: The sealing ability of calcium phosphate silicate-based sealer is superior to that of both pure tricalcium silicate-based and tricalcium silicate- and resin-based sealer