Purpose: This study aims to synthesize copper nanoparticles (Cu-NPs) and evaluate the antimicrobial activity of the plant Hemigraphis colorata. Aim & Objective: The primary objective was to synthesize green Cu-NPs mediated by the leaf extract of the medicinal plant Hemigraphis colorata. Integrating the bioactive compounds of this plant with Cu-NPs is expected to offer significant benefits for various ailments. The biogenically synthesized green Cu-NPs were characterized using UV-visible spectroscopy, FT-IR, XRD, SEM, TEM, and EDAX techniques. Additionally, the antimicrobial properties of the green Cu-NPs were assessed against gram-positive and gram-negative bacteria. Methodology: Hemigraphis colorata (commonly known as murikooti) is a prostrate herb with rooting branches, deep reddish leaves, and white flowers, belonging to the Acanthaceae family. Leaves were collected from a medicinal garden and used for nanoparticle synthesis. The nanoparticles were characterized using UV-vis, FT-IR, XRD, SEM, TEM, and EDAX techniques. The antimicrobial activity of the synthesized nanoparticles was also evaluated. Results and Discussion: Green synthesis of Cu-NPs was achieved using copper sulfate as the precursor and plant leaf extract as the reducing and capping agent. The color change from blue to light brown visually indicated the formation of Cu-NPs. The synthesized nanoparticles were purified by washing with deionized water and ethanol to remove any impurities.