This study aims to assess the antidiabetic effects of the hydroethanolic extract of Artemisia amygdalina leaves in rats with streptozocin induced diabetes. Two dosages, 200 mg/kg and 400 mg/kg, of the hydroethanolic extract were administered orally to evaluate its antidiabetic activity. The results demonstrate a significant reduction in elevated blood glucose levels in both the extract-treated and standard groups. Additionally, a notable increase in animal body weight was observed. Consequently, it can be concluded that the hydroethanolic extract of Artemisia amygdalina exhibits significant antidiabetic properties in Wistar rats, potentially due to the presence of flavonoids. The extract also showed a significant protective effect against streptozocin induced diabetes in a dose-dependent manner.