The present study was conducted to deals with the diversity of Arbuscular Mycorrhizal Fungi in the rhizosphere soil of Linseed cultivars namely ALSI-2, PKV-NL-260 and JLS-73 from drought prone area of Satara district (M.S), India. Linseed (Linum usitatissimum L.) is one of the important rabbi oilseed crops of India belongs to Linaceae family and genus Linum. In India, Linum is grown primarily for Linseed oil which is high source of omega-3 fatty acid not only used for human consumption but also for commercial use. The results revealed variation in soil pH, Arbuscular Mycorrhizal Fungal Spore number, root colonization percentage (%) and Arbuscular Mycorrhizal fungal species in different sampling sites and varieties. Maximum numbers of AM Fungal spores were 105 per 50 gm soil and highest root colonization percentage 86.66 % was recovered from rhizospheric soil from variety PKV-NL-260 with soil pH 8. In general, five species of AM Fungi belonged to four genera viz, Glomus, Acaulospora, Scutellospora and Gigaspora were characterized, being those of the genus Glomus was most predominant in rhizosphere soil of Linseed in all the sites and varieties. In conclusion, the study provided useful information to understand the AMF species in Linseed soil and revealed that soil pH, drought condition (high temperature) and geographical distribution were triggered AMF diversity and spore count in the rhizosphere of Linum.