Green synthesis is an easy, safe, affordable and environmentally beneficial method of creating nanoparticles. In this present study aqueous extract of Carica papaya leaves was used as a capping agent to prepare photosynthesized TiO2 nanoparticles. The formation of TiO2 nanoparticle was confirmed by taking UV-Visible spectra which shows a maximum absorbance in the range of 314nm.The synthesized TiO2 nanoparticles were characterized using XRD, SEM, SEM-EDAX to determine the size, surface morphology, elemental composition. The XRD data revealed that the nanoparticles prepared was to about 19.79nm in size, SEM images predicts that TiO2 nanoparticles are spherical and EDAX spectra confirms the presence of Titanium and Oxygen. Anti-inflammatory activity reveals that biosynthesized TiO2 nanoparticles exhibit good inhibitory activity than the standard used. The synthesized nanoparticles showed dose dependent cytotoxicity towards MCF-7 cell line and shows an IC50 value of 42.59 µg/ml.