The present study aimed to prove that enriched papaya extract as an anti-thrombocytopenic drug, synergetic and antagonistic effect on efficacy of cisplatin against MCF-7 cell progression. MTT test was carried out to test the cytotoxicity of enriched papaya extract against the MCF-7 cell lines. Inhibitory efficacy of enriched papaya extract alone and in presence of cisplatin on MCF-7 cells was observed to analyze the antagonistic and/synergetic effect. DAPI, PI and Green fluorescence strains were used to observe the morphology and nucleus of the cells. BAD gene, an proapoptotic gene expression (qualitative) was carried out by using PCR and agarose gel electrophoresis to observe its gene expression in untreated, treatment with cisplatin and papaya extract in MCF-7 cell lines. The present study revealed that papaya extract don’t exhibit significant inhibition/progression against MCF-7 Cells. Even it did not affect the efficacy of cisplatin against MCF-7 cell progression. The PCR results revealed that BAD genes expression was happened in cisplatin and papaya + cisplatin treated and did not get clear bands in untreated and papaya alone treated cells. Thus concluding that enriched papaya extract might be useful in management of thrombocytopenia induced by cisplatin without any interactions.