IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

Screening Of Total Phenolics, Antihemolytic, Antioxidant Properties, And Gc-Ms Profiling Of Extracts Of Annona Squamosa L. Leaves.

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Sonawane S.R.

Abstract

The present study was carried out for the identification of phytoconstituents present in Annona squamosa L. leaves and also to determine the total phenolic content, and antioxidant and antihemolytic properties of leaf extract. The total phenolic content of the methanolic extract was carried out by using Folin Ciocalteu method which was 256.99±8.78 (mg/g GAE). The antihemolytic activity carried out on rat erythrocytes revealed 87.76% percentage inhibition activity (IC50 163.40µg/mL). The extract of Annona squamosa L. was found to have the most potent anti-oxidant properties in DPPH and H2O2 radical scavenging methods. IC50 value DPPH and H2O2 inhibition of extract were 189.39 µg/mL and 219.01 µg/mL respectively. The GC-MS study was also carried out and which showed the presence of phytochemicals like Neophytadine (RT: 24.39), 2-Pentadecanone, 6,10,14-trimethyl-(RT:21.74).

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