IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

Identification of Zolpidem by Thin Layer Liquid Chromatography

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Bhupendra Singh

Abstract

An elite fluid chromatographic interaction with brilliant recognition for simultaneous evaluation of specific benzodiazepines (BZDs) is created for legal screening of contaminated non-cocktails. The cases were evaluated after a necessary cycle of pH adjustment and separation. It was done at 45oC with a variable advance of 15mM phosphate support: methanol (50:50 v/v) at a stream rate of 1.4 mL/min on a C18 segment (250 mm × 4.6 mm, 5m). An Ultra Violate (UV) detector set to 245 nm was utilized to assess the column eluent. The eluting peaks were quickly found, recognized, and measured as a result of this. Calibration curves for all medications in the 0.510 μg/ mL range with a linear regression coefficient greater than 0.996. The BZDs exhibited recovery rates that ranged from 93.7 to 108.7 percent. In addition, the detection limits were 0.03-0.05 g/mL. The detection limits were determined to be between 0.01 and 0.02 μg/mL. For all BZDs at all focuses in the range of 0.45 to 7.69 percent, the coefficients of differentiation between and between days were resolved. The method will provide an unmistakable, responsive, and quick way for screening six BZDs in contaminated sodas in legal evaluation.

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