Aqueous extract of Azadirachta indica is utilized as reducing agent for the eco-friendly synthesis of Ag-Nps. The nanoparticles were synthesized and characterize utilizing UV–vis, X-raydiffraction (XRD) and FTIR analysis. Crystallinity of the Ag-Nps is proved from the XRD pattern and Scanning Electron Microscopy (SEM). Bi-molecules accountable for capping are a variety of Ag-Nps as established by the FTIR spectra. The temperament of Ag-Nps synthesized to all examined by UV- vis spectra. The Ag-Nps were by means of a regular size of 7–15 nm and typically spherical exact by XRD pattern. The antibacterial activity of synthesized Ag-Nps was assessed with that of aqueous Azadirachta indica by diffusion technique. The Ag-Nps from Azadirachta indica sample prominently reserved bacterial development against multi drug resistant to the entire pathogens which is utilized in this investigation. Thus Ag-Nps displayed wide range antibacterial activity at lower attention and may be an excellent option therapeutic move toward in future.