To improve strategies towards the management and protection of aquatic biodiversity of water bodies, there is a need to collect information on the diversity present at different locations of aquatic habitats. Hence, the biodiversity of the cladoceran fauna of Kolavoi Lake, Chingleput was studied through weekly sampling programme advocated . Remarkably rich cladoceran community consisting of 10 species were recorded. Macrothrix spinosa and Alona quadrangularis were abundant whereas Illyocryptus spinifer and Bosmina longirotris were occurred rarely. During summer season, the diversity of organisms was low. Ceriodaphnia cornuta and Macrothrix spinosa were present throughout the year. Alona macronyx and Illyocryptus spinifer were recorded during monsoon season only. Diaphanasoma excisum and Alona quadrangualaris were observed during summer season. The quantitative and qualitative variability of Cladocerans recorded in Kolavoi Lake is not only attributed to seasonal changes but also with the locations.