Diabetes mellitus is a disorder that occurs when there is little or not enough insulin production from the pancreas. The onset and progression of long-term complications in diabetes mellitus appear to be related to the degree of hyperglycemia and the overall metabolic control. The core remedy for managing diabetes is to lower hyperglycaemia and reduce intestinal glucose absorption through the inhibition of carbohydrate metabolizing enzymes viz. alpha-amylase and alpha-glucosidase. Moringa oleifera is a medicinal plant, and in traditional medicine, it is known as the miracle tree because all the plant parts viz. leaves, seeds, bark, roots, and flowers have nutritional and medicinal uses. Thus, we aimed to assess the antidiabetic potential of aqueous (aq.) leaf extract of Moringa oleifera through in-vitro experimental methods such as inhibition of carbohydrate hydrolyzing enzymes namely, alpha-amylase and alpha-glucosidase. Results depicted that aq. leaf extract of M. oleifera at a concentration range of 20µg/mL, 40µg/mL, 60µg/mL, and 80µg/mL, shown alpha-amylase inhibition effect of 33.20%, 54.12%, 69.31%, and 79.34% respectively with an IC50 value of 61.26 µg/mL in comparison with the standard antidiabetic drug acarbose with an IC50 value of 69.58 µg/mL. Furthermore, aq. leaf extract of M. oleifera at a concentration range of 20µg/mL, 40µg/mL, 60µg/mL, and 80µg/mL, shown inhibition effect of 27.72%, 42.58%, 58.87%, and 71.30% respectively with an IC50 value of 51.62 µg/mL in comparison with the standard antidiabetic drug acarbose with an IC50 value of 64.46 µg/mL. Moreover, quantitative estimation of phytochemicals in aq. leaf extract of M. oleifera revealed total flavonoids quantity was found to be highest (0.21 mg RE/g extract) in aq. leaf extract of M. oleifera followed by total phenolics (0.12 mg GAE/g extract) and total tannins (0.06 mg TAE/g extract). In conclusion, results from this study delineated that aq. leaf extract of M. oleifera possess in-vitro alpha -amylase and alpha-glucosidase activities, and hence it could be presumed from the findings of this study that the crucial mechanisms of anti-diabetic action of leaf parts of M. oleifera is via inhibition of carbohydrate inhibiting enzymes such as alpha- amylase and alpha-glucosidase.