Vegetables are well known for their nutritional and medicinal properties but vegetables also contain some antinutritional factors which can affect the availability of the nutrients. The present study aims to assess the anti-nutritional factors of some selected vegetables collected from the 2 different Ghats of Prayagraj district namely Phaphamau and Bakshi bandh. The Vegetables selected for this study were Spinacia oleracea, Coriandrum sativum, Trigonella foenumgraceum, Cucurbita, Lagenaria siceraria, Cucumis sativus, Solanum lycopersicum, and Beta vulgaris. These vegetables were assessed chemically for the presence of oxalate, Phytates, tannins and alkaloids. The anti-nutritional factors of vegetables were analysed by using standard methods. The results ranged as follows; oxalate (5.4±0.3-664±1.2 mg/100g), Phytates (2.4±0.2- 44.2±0.6 mg/100g), Tannins (5.5±0.5-22.3±0.47 mg /100gm), and alkaloids (3.7 ±2.6- 17.2 ±0.23%).Though these anti- nutrients can interfere with nutrients utilisation when in high concentration, the values obtained for the vegetables analysed were not up to the toxic levels of the anti-nutrients. Application of cooking methods in a significant way is accountable for reducing the risk of ANF in the human body