Review of HPLC Method for Assessing the Purity Profile of Chosen Antiviral and Anticancer Medication

Abstract

High-Performance Liquid Chromatography (HPLC) is a pivotal analytical technique extensively employed in pharmaceutical industries to ascertain the purity of drugs. This review critically examines various HPLC methodologies employed for assessing the purity profiles of selected antiviral and anticancer medications. The review emphasizes the importance of selecting appropriate chromatographic conditions, including mobile phase composition, column type, and detection wavelength, to achieve optimal separation and accurate quantification of drug components. Various validation parameters such as linearity, accuracy, precision, specificity, and robustness are also discussed, highlighting their significance in ensuring the reliability and reproducibility of HPLC results. case studies and comparative analyses of different HPLC methods used for specific antiviral and anticancer drugs underscore the diverse approaches employed in pharmaceutical analysis. The review also addresses current trends and advancements in HPLC technology, including the integration of advanced detectors and automation, which contribute to enhanced sensitivity and efficiency in drug purity profiling. this review consolidates current knowledge and practices in HPLC methodology for assessing drug purity, offering insights into method development, validation strategies, and technological innovations. It serves as a valuable resource for researchers and pharmaceutical professionals seeking to optimize HPLC methods for ensuring the quality and efficacy of antiviral and anticancer medications.