ISOLATION AND IDENTIFICATION OF BACTERIA FROM TODDY AND THEIR EVALUATION FOR PROBIOTIC NATURE AND ANTIMICROBIAL ACTIVITY

Abstract

Probiotics are health friendly bacteria found in fermented foods and cultured milk. They have a variety of health benefits such as bowel disease prevention, enhancement of immune system, lactose intolerance, modulation of gut-brain, antihypercholesterolemic and antihypertensive effects, alleviation of postmenopausal disorders and reduction of traveler's diarrhoea. Many studies had been focused on their use for the treatment of skin and oral diseases. With the current focus on disease prevention and the quest for optimal health at all ages, the probiotics market potential is enormous. Fresh unfermented toddy is very sweet and non-alcoholic. Palm sap begins fermenting immediately after collection, due to natural microbes from the air. The present study aims to isolate & characterisation of the bacteria from white liquid collected from the palm trees and their identification by 16s rRNA sequencing . The identified bacteria was named as M1 and sequencing results showed that the bacteria as Staphylococcus hominis and was evaluated for acid tolerance test, antimicrobial activity to indicate the probiotic nature .