ASSESSMENT OF IMPROPERLY STORED TETRA PACK FOODS FOR THEIR MICROBIOLOGICAL QUALITY AND SAFETY

Abstract

The improper storage of Tetra Pack foods poses significant risks to their microbiological quality and safety, potentially compromising consumer health. Samples were subjected to controlled storage conditions mimicking improper handling, followed by microbiological analysis to quantify total viable counts (TVC), This study assessed the microbiological quality and safety of seven Tetra Pack food samples (Mango Juice, Milk, Soya Milk, Buttermilk, Flavoured Milk, Tomato Puree, and Coconut Milk) exposed to environmental conditions for 8 days. Samples were analyzed periodically on the 2nd, 4th, 6th, and 8th days to evaluate contamination levels. Total colony counts increased progressively over time, with 45 bacterial isolates identified. Among these, 4 were gram-positive cocci (9%), including Staphylococcus aureus (75%) and coagulase negative Staphylococci (25%); 21 were gram-negative rods (47%), and 20 were gram-positive rods (44%). Predominant pathogens included Enterobacter aerogenes, Kluyvera ascorbata, Salmonella enterica, Pseudomonas aeruginosa, and Bacillus species. Specific pathogens varied by product: Mango Juice contained E. aerogenes, Bacillus coagulans, and S. aureus; Milk harbored K. ascorbata, Bacillus simplex, and P. aeruginosa; and Tomato Puree showed S. enterica, B. coagulans, and coagulase-negative Staphylococci. Additionally, five crunchy snacks and five beverages were tested for non-permitted food colors. Metanil yellow was detected in three snacks, and fast red dyes were found in two beverages, indicating the use of unauthorized additives.