The aim of the present research was to develop a bilayer tablet of Pregabalin Using Modified Natural Polysaccharide. The present research was aimed to develop sustained release mini tablets of Pregabalin by direct compression method. Because of its low solubility and bioavailability, the formulation of pregabalin presents difficulties; the drug is extensively used to treat neuropathic pain and seizures. Using a modified natural polysaccharide as the main excipient, our goal in this work was to create a bilayer tablet formulation of pregabalin. The biocompatibility, controlled release, and ability to increase medication solubility of the modified natural polysaccharide were among the desired attributes that led to its selection. Two layers of pregabalin were used in the bilayer tablet: one for immediate-release, which would have an effect quickly, and another for sustained-release, which would keep therapeutic plasma levels steady for a long time. Similar to the intended pharmacokinetic profile of pregabalin, in vitro dissolution investigations showed that the bilayer tablet formulation produced a biphasic release profile, with an initial fast release phase followed by sustained release. Research on the stability of the bilayer tablets showed that even when subjected to rapid storage, they retained all of the drug content and release properties.