In recent years, nanotechnology has experienced explosive growth. Because of their potential for attaining targeted processes and selectivity, nano-structured materials are garnering a lot of interest, particularly in the fields of biology and medicine. Nanotechnology has become one of the most practical technologies, because of unique physical and chemical features of nanoparticles. With their dazzling and intriguing optical, dielectric, and photo-catalytic features resulting from size quantization, nanomaterials like titanium dioxide nanoparticles (TiO2-NPs) have become a new generation of sophisticated materials. Applying Nanoparticle-based formulations to their media prevents the growth of resistant bacteria. The ability of some nanoparticles to inhibit the growth of bacteria makes them promising candidates for use as antimicrobial agents. Disk diffusion technique is used to analyze the antibiotic resistance pattern of E. coli and can be performed with both liquid and agar nutritional media. The bactericidal efficacy of 0.01, 0.5, 1, and 1.5% nano-TiO2 was determined using the optical density (OD) and the Kirby-Bauer disc diffusion test. Antibiotic resistance was observed for this strain across the board. Optical density decrease was detected with nano-TiO2 concentration increase (0.225, 0.218, 0.158, 0.075, 0.031respectively).