Agaricus bisporus (J. E. Lange) Imbach is one of the most cultivated edible mushrooms in India. In the present study, antibacterial activities of acetone and methanol extract of A. bisporus were evaluated against Bacillus cereus, Staphylococcus aureus, Salmonella typhi, and Escherichia coli through the disc diffusion method. Antioxidant potential was evaluated through reducing power, DPPH scavenging activity, total phenol contents (TPC), superoxide dismutase activity (SOD), ascorbate peroxidase activity (APX) and non-protein thiol contents (NPT).The zones of inhibition (ZOI) showed by acetone extract were7.73 mm to 9.44 mm and methanol extract was6.46 mm to 6.61mm. The reducing powers of acetone and methanol extracts were 0.196 and 0.272 respectively, the IC50 values of DPPH scavenging activities of acetone and methanol extracts were 1132.79 μg/ml and 381.03 μg/ml respectively and TPC of acetone and methanol extracts were 1.815 μg GAE/mg DW and2.775 μg/mg DW respectively observed. The SOD activity of 10.30 ± 0.33 U/mg protein, the APX activity of 2.58 ± 0.26 μ moles/mg protein min-1, and NPT contents 1.413 ± 0.040 μ moles/g DW were observed in A. bisporus. The results of this study emphasize further isolation and characterization of natural antibacterial substances and antioxidants and their use in the medicine and food industries.