IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF A VERSATILE NUTRACEUTICAL: CURCUMIN

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Syeda Ayesha

Abstract

Nutraceuticals are products, which other than nutrition is also used as medicine. A nutraceutical product may be defined as a substance, which has physiological benefit or provides protection against chronic disease. Simple, sensitive, selective, rapid and reliable methods for the spectrophotometric determination of curcumin belonging to the class of neutraceuticals has been worked out. The methods are based on the reaction of curcumin with folin ciocalteu (FC), an electrophilic coupling agent in presence of iron (III) in mild hydrochloric acid medium. The bluish –green complex shows maximum absorbance at 650nm. The colour complex can be extracted into chloroform. The methods obey Beer’s law. As many as 10 anions and cations do not interfere. The methods have good reproducibility and can be satisfactorily applied to the determination of curcumin in various turmeric samples.

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