IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

SCREENING OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF CARICA PAPAYA PLANT LEAF AND SEED EXTRACT

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Lini, J.J. and Selva Shamal, V.P.

Abstract

The present study was designed for the evaluation of phytochemical and antioxidant activity of carica papaya leaf and seed extracts. Bioactive compounds of the leaves and seed of papaya were extracted using petroleum ether, chloroform and ethyl acetate for phytochemicals investigation. Most of the the extract revealed the presence of alkaloid, flavonoid, phenol, carbohydrate, tannin, glycosides, saponin, anthraquinine, protein and aminoacids. The carbohydrate and phenol content value were maximum. The results of free radicle scavenging potential of the different extracts tested by DPPH assay, a stable free radicle has charecteristics of absorption at 695 nm. The reduction capability of the DPPH radicles was determined by the decrease in absorbance at 695 nm, where a change in colour to yellow denotes quenching of free radicles by plant extracts. DPPH radicles react with suitable reducing agents, during which the electrons become paired off and the solution losses colour depending on the number of electrons taken up. Highest activity was exerted in ethyl acetate extract of papaya. Papaya seed has more total antioxidant activity than leaf.

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