Diabetic neuropathy is the most dangerous complication of diabetes which is very difficult to treat. Its diagnosis in the early stage is very essential for its control and prevention of the illness. The protective effect of hydroethanolic extract of Clematis buchananiana leaves were investigated for evaluation of the neuroprotective effect in diabetic-induced neuropathy in Wistar rats. Streptozotocin induces diabetes within 3 days by destroying the beta cells of the pancreatic gland. For the assessment of diabetes, blood glucose level was measured with the help of a glucometer. Two different pain models i.e., Eddy’s hot plate and Tail flick analgesiometer were used for the measurement of analgesic activity in Wistar albino rats. As a result of the study, it was discovered that the animals treated with a standard drug (Gabapentin, 100mg/kg) had the maximum neuroprotective effect, followed by the higher dose of hydroethanolic extract of C. buchananiana leaves. After this study, it can be concluded that hydroethanolic extract of C. buchananiana has exhibited a protective effect in diabetic-induced neuropathy and it can be further explored and investigated for benefits of humans in the management of several ailments.