The aim of the present study is to analyze the phytochemical and antibacterial activity of Cissus quadrangularis L. collected from different regions of Vadakkankulam, Tirunelveli District, Tamil Nadu. Aqueous extracts and n-butanol extracts along with the leaves of C. quadrangularis L. shows the presence of tannins, steroids, carbohydrates, saponins, terpenoids, alkaloids, phenols and flavonoids. Glycosides and Steroid compounds were found to be absent in the leaves extracted with aqueous. Flavonoids and phenols compounds were found to be absent in the leaves extracted with n-butanol. Aqueous and n-butanol were used for extraction and samples were tested against different group of microorganisms like Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Enterobacter. The results of the study indicated that the aqueous leaf extract possessed most potent antibacterial activity as compared to n-butanol leaf extract.