Growing global awareness of sustainability concepts has made it more popular in recent years to transformation from biological waste to biological resources using sustainable chemistry also use them to produce goods. This article describes a safe method for simultaneously extracting keratin and extracting individual’s hair melanin with an ionic liquid [BMIM]Cl, also known as 1-butyl-3-methylimidazolium chloride. The recovered protein was thoroughly examined through chemical characterisation, secondary structure investigations, and thermal analysis. Blood-contacting biomaterials, including sealants, catheters, haemostats, scaffolds for tissue engineering, and the like, can use keratin since haemolytic potential testing showed that it is haemocompatible. The ellipsoidal form of melanin was preserved during the extraction process, as demonstrated by scanning electron microscopy. 2,2-diphenyl-1-picrylhydrazyl indicative was shown to be reduced by the pigment.