Mimusops elengi also known as Spanish cherry is commonly used in traditional medicine for various purposes. Mimusops elengi leaves extracts possess antioxidant and anticancer properties. The study was conducted to explore the use of leaves of M. elengi to evaluate the scientific basis of cytotoxic and anti-tumour activity. Phytochemical analysis of Mimusops elengi has identified the presence of several bioactive compounds, including alkaloids, flavonoids and saponins. These compounds have been shown to exhibit various pharmacological activities, including antioxidant, antibacterial, antifungal, and anticancer properties. Radical scavenging activity of the methanol extract was found to be around 78% in DPPH Assay and 92% in ABTS assay. Isolation of bioactive compounds were done using thin layer chromatography followed by column chromatography. UV-Vis Spectroscopy, FT-IR and GC-MS served as tools for characterization and to find the high concentration of bioactive compound. Benzoate ester was found in highest concentration in the 90% EA and 10% M fraction from column chromatography. Mimusops elengi extracts have been shown to induce apoptosis (programmed cell death) in cancer cells. In vitro anti-tumour potential was evaluated by using the bioactive compound obtained in Breast cancer cell line (MDA-MB-231 and it showed effective results with minimal side effects on the normal cells. The treated cells were subjected to fluorescence microscopy based Acridine orange/ Propidium iodide (AO/PI) staining to determine apoptosis induction. The main objective was to obtain a plant-derived alternative/ Chemo-preventive drug for breast cancer cell line so as to obtain high cytotoxicity and reduced side effects. With Molecular docking, the interaction of the bioactive compound and breast cancer cell surface protein, Progesterone Receptor (PR) were studied.