Medicinal plants are very interesting, began to focus on discovery of natural products as potential active principles against various diseases. In this study proposes to evaluate the antibacterial and larvicidal activities of selected plant Biophytum sensitivum (L.). The antibacterial activities were determined against human pathogens viz Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Proteus vulgaris. The highest value observed from ethanol extracts of Biophytum sensitivum (L.) showed antibacterial activity against Escherichia coli. All the tested human pathogens were highly sensitive to ciprofloxacin. The N-butyl alcohol extracts of Biophytum sensiivum showed best result of larvicidal activity against the mosquito larvae Aedes aegypti. This study Biophytum sensitivum in the traditional system of to treat various infectious diseases, caused by the microbes as well as in mosquitos.