Drinking water containing F- >1.5 mg/l is recognized as a major health problem. In fact, the Fconcentration <1.5 mg/l is an essential trace element for human health, but, in excessive intake may cause fluorosis. The present study used the novel adsorbent limonite for the investigation of the defluoriation capacity in aqueous condition by fixed-bed column. The potential of defluoridation of limonite was done under variable operating conditions such as pH, inlet Fconcentration, bed height and flow rates. At pH 5.02 the maximum adsorption of Fonto the surface of the limonite observed. The column studies showed that the adsorption capacity of the bed decreased with increase of flow rate and inlet Fconcentration. The FTIR spectra of limonite shows that it contains –OH group which might be act as ion exchange for Fion. SEM and XRD images also give good evidence of the adsorption of F- on the surface of limonite. The Breakthrough curve Models, Thomas Model and Yoon-Nelson Model were giving a good connection with experimental results. The column capacity for the actual high F- water was calculated 13.4 mg/g.