Lallemantia royleana is a member of the Lamiaceae or Labiateae family.Its seeds were used as cooling, diuretic, and sedative; as a soothing agent during urinary troubles, fever, common cold, intestinal troubles. It was also used as cephalic astringent, cardiac tonic, carminative and for cough.Using a Soxhlet apparatus, methanol was used to extract the Lallemantia royleana seeds. The resulting dried powder was then mixed with DMSO at a concentration of 1 mg/ml. By employing the well diffusion technique, these methanolic extracts were assessed for their antimicrobial activity. The test was run on various fungus and bacterial strains. Five fungal strains and ten bacterial strains were examined for antimicrobial activity. In the current investigation, a crude methanolic extract made from Lallemantia royleana seeds was used.Various doses of the methanolic seed extract revealed various-sized zones of inhibition. The methanolic extracts of Lallemantia royleana showed the most promising anti-bacterial activity against Gram-positive Staphylococcus epidermidis and S.aureus. Also, gram-negative bacteria like Proteus vulgaris showed great antibacterial action. The current study additionally assessed the crude methanolic extract of Lallemantia royleana seeds' anti-fungal properties. It was observed that it significantly inhibited the growth of Candida albicans and Saccharomyces cervisiae. L. royleana's crude methanolic seed extract can be used to treat human pathogenic illnesses as a powerful antibacterial agent. In order to effectively combat fungal diseases, L. royleana's crude methanolic seed extract can be employed.