A set of three disomic substitution lines for chromosome 1D of Aegilops tauschii developed in 1990 in Triticum durum cv. PBW114 background carried genes for red glume color. Out of three, two lines (1D3597 and 1D3601) had a substitution of chromosome 1D(1A) and one line (1D3598) had a substitution of chromosome 1D(1B). The SDS-PAGE analysis of the disomic substitution line for chromosome 1D(1A) showed the presence of HMW-GS subunit of 2t + T2 and 6+8 belonging to 1D, 1B chromosomes and substitution line for chromosome 1D(1B) showed the presence of 2t + T2 glutenin subunit belongs to 1D chromosome. Micro SDS-sedimentation test analysis of disomic substitution lines (3597 and 3601) for chromosome 1D(1A) had increased nearly two-fold in SDS-sedimentation value than to the parent durum cultivar and substitution line 3598 for chromosome 1D(1B) slightly decreased than to the parent durum cultivar PBW114. The present study describes the interactions of 1D chromosomes in the Triticum durum cultivar PBW114 and their effect on bread-making quality. The analysis suggests that chromosome 1B is a slightly more important subunit than chromosome 1D and in the combination of both chromosomes 1B and 1D important allele to improve bread-making quality in durum wheat.