Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. In the past two decades, many valuable bioactive compounds with antimicrobial, insecticidal, cytotoxic, and anticancer activities have been successfully discovered from Natural compounds. Currently, a few of them are being used to treat cancer. Some endophytes fungi are have the ability to produce the same or similar bioactive compounds as those originated from their host plants. Camptothecin (CPT), the third largest anticancer drug, is produced mainly from Camptotheca acuminata and Nothapodytes foetida. In the present study is focussed to develop Camptothecin from endophyte Fungi and to elevate their potential anti-cancer properties against Hela cervical cancer cells. Cervical cancer cells were treated with the isolated camptothecin and its effect was analysed using the cell cycle analysis. From the investigation the cytotoxic properties against cervical cancer and anticancer activities have been successfully discovered from endophytic fungi. Thus appears to display potent antitumor activity against human cancer via the induction of apoptosis, and may be a useful to develop alternative drug for different cancer therap.