The commercial medication known as Gaozaban contains a significant amount of ARNEBABENTHAMII, which possesses antibacterial, antifungal, anti-inflammatory, or wound-healing effects. An inside vitro anticancer activity for Arnebiabenthamii leaf extract was examined in the current study. Cytotoxicity of theextracts (10–100 µg/mL) was tested on human colorectal cancer cell line, HCT 116.The purpose of this study has been to determine whether a methanolic extract of A. benthamii leaves might beused to treat colon cancer cells from the HCT 116 cell line. GC-MS was used to characterise the extract'sphytochemical properties. The MTT assay was used to detect the extract's effect on cell growth. An annexin VFITC labelling was used to determine the commencement of apoptosis, The potential of cell migrationinhibition was examined using a wound healing assay. Cell proliferation was inhibited by methanolicextract of A. benthamii leaves when examined using MTT cell viability assay in a dosage and timedependent manner. In addition, treatment with methanolic leaf extract of A. benthamii inhibited the cells'capacity to colonise and induced cell cycle arrest. Our results suggest that crude extract from the leaves of A. benthamii plays a significant role in abrogating cancer proliferation by inducing cell cycle arrest &down regulate the metastatic properties in colorectal cancer cells and hence, it may therefore, bedeveloped into potential chemopreventive drug against colorectal cancer in the near future.