The global aquaculture industry heavily relies on the production of Litopenaeus vannamei, commonly known as the whiteleg shrimp, due to its economic significance and widespread consumer demand. However, the industry faces persistent challenges from various pathogens, among which Enterocytozoon hepatopenaei (EHP) stands out as a significant threat to shrimp farming. EHP infections have been associated with reduced growth rates and increased mortality in L. vannamei, posing substantial economic losses and sustainability concerns for shrimp producers worldwide. This study aims to investigate the impact of EHP infection on the differential growth patterns of L. vannamei through comprehensive Polymerase Chain Reaction (PCR) studies.