Epilepsy represents a complex neurological disorder characterized by abnormal electrical activity in the brain, often leading to detrimental effects on cellular processes. This original research article investigates the toxicity of epilepsy on brain tissue by focusing on the activities of crucial enzymes, including Total ATPases, Na⁺ K⁺ ATPases, Mg²⁺ ATPases, and Ca²⁺ ATPases in Wistar strain male albino rats. Additionally, the study explores the modulatory effects of coumarin, a naturally occurring compound with potential therapeutic properties. The experimental design involves the induction of epilepsy in the animal model and subsequent evaluation of ATPase activities in the presence and absence of coumarin. The findings reveal alterations in ATPase activities associated with epileptic conditions, shedding light on the biochemical changes occurring in brain tissue. Furthermore, the investigation explores the potential neuroprotective effects of coumarin, providing valuable insights into its role as a modulator of ATPase activities in the context of epilepsy-induced toxicity. This research contributes to our understanding of the molecular mechanisms underlying epilepsy-related alterations in brain tissue and suggests avenues for further exploration of coumarin as a potential therapeutic intervention for mitigating these effects.