Background: It has been seen during the COVID times that hand hygiene plays a very important role in prevention of it’s transmission. A health care worker plays a very important role in contact transmission of infections. Awareness among the students of allied health Sciences can help aid in reducing the infections. Thus a study regarding assessment of the knowledge, attitude and practices of hand washing during Covid -19 pandemic was undertaken in first year students of Allied Health Sciences. Methodology: A cross sectional study was undertaken with students of allied health sciences to assess their knowledge, attitude and practices regarding hand hygiene. The students were trained regarding hand hygiene and a pre training and post training questionnaire was given and assessed. Result: Total sixty-two students of GNM and DOTT course participated in the study. There was a significant improvement in knowledge, attitude and practices of the students after training sessions. Conclusion: The novel students of allied health sciences were probable targets of imparting hand hygiene knowledge. The interventional change in attitude and practices of the students at grass root level will revolutionise the infection control practices in any health care facility.