Biosensor development is largely dependent on the creative synthesis and careful engineering of conductive polymers with remarkable mechanical and electrical characteristics. Because of their exceptional qualities of high conductivity, environmental stability, and biocompatibility, conductive polymers—in particular, polypyrrole (PPy)—have drawn a lot of interest as potential candidates for biosensor applications. This work investigates the use of electrophoretic deposition (EPD), a method well-known for its capacity to provide consistent and well-adhered coatings on a variety of substrates, in the creation and thorough characterization of nano-structured PPy films