The present study suggests that Indian niger seeds have potential bioactivities. The inherent bioactive molecules may contribute to the stability and radical quenching ability of niger seed oil and cake. The extractability of these bioactive molecules can be increased by the use of polar solvents. Methanol proved to be a good solvent for extraction of both oil and cake and hence revealed the source of antimicrobial activity. Further studies can be made to identify the bioactive principles and also to exploit the products of this widely distributed weed species.