Enterocytozoon hepatopenaei (EHP) infection poses a significant threat to Litopenaeus vannamei aquaculture, impacting shrimp health and productivity worldwide. Understanding the relationship between EHP infection and differential growth patterns is crucial for developing effective disease management strategies. This study explores the association between EHP infection intensity and growth dynamics in L. vannamei using Polymerase Chain Reaction (PCR) profiling. Through controlled experiments and molecular analyses, we investigate the molecular mechanisms underlying differential growth patterns in response to EHP infection. Our findings provide insights into the complex interplay between EHP infection, molecular responses, and shrimp growth performance, offering implications for disease control and sustainable shrimp production.