Volume 13 | Issue 4
Volume 13 | Issue 4
Volume 13 | Issue 4
Volume 13 | Issue 4
Volume 13 | Issue 4
In this study, we evaluated the capacity for somatic embryogenesis and plantlet regeneration in four elite genotypes: P-1 (240D X 281D), P-2 (80D X 281D), C-1 (98C X 254D), and C-2 (98C X 208D). To initiate callus formation and proliferation, zygotic embryos (ZEs) were cultured on N6 media supplemented with 2 mgL-1 Dicamba for a duration of 90 days. Following induction, embryogenic calli were cultured for 120 days in N6 media containing 0.1 mgL-1 2,4-D, 0.16 mgL-1 putrescine, 0.5 mgL-1 casein, and 2.0 g/L activated charcoal to promote somatic embryogenesis and maturation. The differentiated polyembryoids were then transferred to regeneration media consisting of N6 with 0.5 mgL-1 NAA, 1.0 mgL-1 BAP, and 0.5 mgL-1 activated charcoal. Among the studied genotypes, P-2 and P-1 exhibited the highest rates of callus induction, embryogenic calli, differentiated polyembryoids, and a greater number of plantlets per somatic embryo cluster. Overall, P-2 and P-1 genotypes displayed more promising results throughout the entire process of somatic embryogenesis and regeneration from matured ZEs of the dura variety.