Biofuel, which may be made from a variety of carbon-based feedstock, may offer a sustainable option in light of the mounting pressure on crude oil sources and the resulting environmental deterioration. In this study, Biofuelis evaluated for its potential as a fuel for C I engines. The purpose of this paper is to examine the efficiency and emissions of a Biofuel&-powered twin-cylinder diesel engine in relation to conventional diesel powertrains. This paper details the experimental setups and procedures carried out to investigate the effects of both fuels on the emission characteristics and fuel consumption of a diesel engine. Before beginning the experiment, thorough preparations were made by studying the experimental apparatus and components in detail. The necessary experimental data for the analysis can be noticed. After collecting all of the necessary data for the thesis, the necessary calculations and analysis have been performed. In the experiment, a C I engine was employed, but it was under no stress