Pomegranate (Punica granatum L.) is an economical and commercial crop of tropical and subtropical India. Among the plant-parasitic nematodes, root-knot nematode (Meloidogyne spp.) is considered to be an economically important pest of pomegranate cultivation in Maharashtra. Almost all pome orchards are noticed to be infested with this nematode and incur a huge monetary loss to the growers. Furthermore, nematode parasitism has observed to increase the tree’s susceptibility to soil-borne pathogens and induce wilt, subsequently tree succumb to death. Nematicides have been recommended so far but have limited use as these nematicides are costlier to the growers, toxic to the environment and polluting bio flora and fauna. In the present study, the root samples of nematode infested pome plants were collected washed and females were isolated from galls.