In the present investigation, it was attempted to establish the prebiotic potential of Mangifera mucilage and also perform the proximate analysis of the extracted mucilage. Mangifera mucilage was extracted from the leaves in yield of 5.1% by weight of dry leaf powder. The mucilage was also evaluated for its DPPH radical scavenging potential and its resistance to hydrolysis by gastric juice (acidic hydrolysis) or by α-amylase (enzymatic hydrolysis). The prebiotic potential of the mucilage was studied by assessing its effect on the growth of various lactobacillus strains. The mucilage was found to contain 16.25% moisture, 4.71% ash, and 71.2% carbohydrates. Proteins and fats were totally absent in the mucilage. The total sugar in the Mangifera mucilage sample was determined by Fehling’s reagent method and was found to be 61 µg. The IC50 value of the mucilage in inhibiting DPPH radical was found to be 43.63 µg/mL. Artificial gastric juice (pH 1) was used to hydrolyze the Mangifera mucilage as well as inulin. The acidic hydrolysis of inulin was found to be 7.14% while that of the Mangifera mucilage was found to be 16.91%. The enzymatic (-amylase) hydrolysis of inulin was found to be 12.34% while that of the extracted Mangifera mucilage was found to be 18.36%. The prebiotic promoted the growth of Lactobacillus strains in varying degree. The highest growth was obtained for L. fermentum (1.71 ± 0.03) followed by L. rhamnosus (1.45 ± 0.02 Log10CFU/mL) and the least for L. acidophilus (1.29 ± 0.03 Log10CFU/mL).