The present investigation was aimed to screen the antimicrobial potential of Begonia albococcinea Hook. against various human bacterial and fungal pathogens. Antimicrobial efficacy was performed by disc diffusion method against the bacterial pathogens viz., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella flexneri, Bacillus cereus, Bacillus subtilis, Enterococcus faecalis., and Staphylococcus aureus incubated for 24 hrs at 37°C as well as the isolates of fungal pathogens Candida albicans, Candida tropicalis, Aspergillus flavus and Aspergillus niger. All the extracts of the plant viz., acetone, methanol, benzene and chloroform investigated exhibited varying degree of inhibitory effect against the selected human pathogens. The investigation reveals that, B. albococcinea forms a potentially good source of antimicrobial agent and demonstrates the importance of such plant in medicinal systems for curing various ailments.